Provided is a guide device for injection needle puncture. The guide device for injection needle puncture according to one embodiment of the present invention comprises: a body; a guide part which has a guide hole provided therein so as to be tilted at a certain angle along the height direction, the guide hole allowing an injection needle to pass therethrough so that during injection needle puncture, the insertion angle of the injection needle toward the human body is guided and the injection angle can be constantly maintained; and a sliding member which is coupled to the body so as to be slidingly movable, wherein the spaced distance between the guide hole and a medical ultrasound apparatus is adjusted through relative movement of the body or the sliding member so that a target location into which the distal end of the injection needle is inserted can be located on an entrance path of the injection needle.