基本信息:
- 专利标题： METHODS AND SYSTEMS FOR CHARACTERIZING ANALYTES USING NANOPORES
- 申请号：EP23182828.6 申请日：2017-11-29
- 公开(公告)号：EP4269617A3 公开(公告)日：2024-01-10
- 发明人： CLARKE, James , WHITE, James , MUSCAT, Richard , KNOTT, Jessica Mary May , NATHANI, Ramiz Iqbal , HERON, Andrew John , BRUCE, Mark John , JAYASINGHE, Lakmal Nishantha , CAPROTTI, Domenico , STODDART, David Jackson , BOWEN, Rebecca Victoria , WRIGHT, Christopher James , MOODY, Paul Richard
- 申请人： Oxford Nanopore Technologies plc
- 申请人地址： GB Oxford Science Park Oxford OX4 4DQ Gosling Building Edmund Halley Road
- 代理机构： J A Kemp LLP
- 优先权： US201762471338 P 20170314
- 主分类号： C12Q1/6869
- IPC分类号： C12Q1/6869
摘要：
Methods of characterizing an analyte using a nanopore. One aspect features methods for characterizing a double-stranded polynucleotide using a nanopore, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.