发明公开
EP4148123A1 IN-VITRO CULTURE, INDUCTION, ACTIVATION AND CRYOPRESERVATION METHOD AND CELL BANK ESTABLISHMENT FOR IMMUNE CELLS
审中-实审
基本信息:
- 专利标题： IN-VITRO CULTURE, INDUCTION, ACTIVATION AND CRYOPRESERVATION METHOD AND CELL BANK ESTABLISHMENT FOR IMMUNE CELLS
- 申请号：EP20934683.2 申请日：2020-05-26
- 公开(公告)号：EP4148123A1 公开(公告)日：2023-03-15
- 发明人： ZHANG, Bingqiang , CHEN, Mengmeng
- 申请人： Qingdao Restore Biotechnology Co., Ltd.
- 申请人地址： CN Qingdao, Shandong 266000 Floor 1 and 2, Building 7, Incubation Center Qingdao Blue Biomedical Industrial Park No. 368 Hedong Road High-Tech Zone
- 代理机构： Cabinet Chaillot
- 优先权： CN202010371435 20200506
- 国际公布： WO2021223274 20211111
- 主分类号： C12N5/0783
- IPC分类号： C12N5/0783 ; C40B50/06 ; A01N1/02
摘要：
The present invention discloses an in-vitro culture, induction, activation and cryopreservation method and cell bank establishment for immune cells. The method includes the follows: using a dedicated amplification medium of immune cells to perform first-stage amplification culture on mononuclear cells to obtain preliminarily amplified immune cells; using a dedicated induction medium of immune cells to perform second-stage induction and amplification culture on the preliminarily amplified immune cells to obtain induced immune cells; using a dedicated activation medium of immune cells to perform third-stage activation and amplification culture on the induced immune cells to obtain a large number of immune cells with activation functions; using a dedicated cryopreserving fluid of immune cells to cryopreserve the immune cells to obtain cryopreserved immune cells; and performing preservation according to ABO/RH typing and HLA typing; and establishing an information file of immune cells for retrieval to construct an immune cell bank.