发明公开
EP4008791A1 MOLECULAR DIAGNOSTIC KIT FOR DETECTING NUCLEOTIDE SEQUENCES AND METHODS FOR DETECTING INFECTIOUS AGENTS USING SAID KIT
审中-实审
转让
基本信息:
- 专利标题： MOLECULAR DIAGNOSTIC KIT FOR DETECTING NUCLEOTIDE SEQUENCES AND METHODS FOR DETECTING INFECTIOUS AGENTS USING SAID KIT
- 申请号：EP20843787.1 申请日：2020-07-22
- 公开(公告)号：EP4008791A1 公开(公告)日：2022-06-08
- 发明人： CARRILLO, Carolina , VOJNOV, Adrian Alberto , LAROCCA, Luciana , STOLOWICZ, Fabiana , WERBAJH, Santiago Enrique
- 申请人： Melossi Jiménez, Andrés , Laboratorio Pablo Cassara S.r.L. , Unifarma S.A. , Consejo Nacional De Investigaciones Cientificas Y Tecnicas (Conicet)
- 申请人地址： CL Santiago, 7500000 Europa 2035; AR Buenos Aires, C1408GBV Carhue 1096; AR Buenos Aires, 1427 Céspedes 3857; AR Buenos Aires, 1425 Godoy Cruz 2290
- 当前专利权人： CONSEJO NACIONAL DE INVESTIGACIONES CIENTIFICAS Y TECNICAS (CONICET)
- 当前专利权人地址： CONSEJO NACIONAL DE INVESTIGACIONES CIENTIFICAS Y TECNICAS (CONICET)
- 代理机构： Durán-Corretjer, S.L.P.
- 优先权： ARP190102073 20190723
- 国际公布： WO2021012063 20210128
- 主分类号： C12Q1/6844
- IPC分类号： C12Q1/6844 ; C12Q1/6888
摘要：
Molecular-based diagnostic kits and methods for detecting nucleotide sequences of DNA, DNA copy and RNA of infectious agents, transgenes, alleles or non-encoding sequences, with no initial isolation are provided. The kit comprises at least a first calibrated dropper bottle with an anti-contamination system containing a solution of polymerase enzyme, chaotropic agents, salts and deoxyribonucleotides; a second calibrated dropper bottle with an anti-contamination system containing a set of at least 4 primers, part of the sequences being preserved in the nucleic acid fragment to be detected; a dropper bottle containing a mixture of developing reagents; a carrier to hold the sample; at least one LFD developing system, colorimetric or fluorogenic reaction; positive and negative controls. The polymerase enzyme is selected from Bst enzymes formed by DNA polymerase/helicase, with no exonuclease activity. The set of primers is designed from gene sequences to be detected, whether of deoxyribonucleic or ribonucleic nature.