An actuation-and-detecting module is disclosed and includes a main body (1), a particle detecting base (2), plural actuators (31, 32) and sensors (41, 42, 43). The main body (1) has a first compartment (1a) divided into a first chamber (11d) and a second chamber (11e), a second compartment (lb) having a third chamber (12c) and a third compartment (1c) divided into a fourth chamber (13d) and a fifth chamber (13e) by a carrying partition (13c). The particle detecting base (2) is disposed between the fourth chamber (13d) and the carrying partition (13c) of the third compartment (1c). The first actuator (31) is disposed between the second chamber (11d) and a first partition (11c). The second actuator (32) is disposed within a receiving slot (22) of the particle detecting base (2). The first sensor (31) is disposed within the first chamber (11d). The second sensor (32) is disposed within the third chamber (12c) for detecting the air in the third chamber (12c). The third sensor (43) is disposed within a detecting channel (21) of the particle detecting base (2) for detecting the air in the detecting channel (21).