发明公开
EP3387134A1 METHODS AND COMPOSITIONS FOR ENHANCED NUCLEASE-MEDIATED GENOME MODIFICATION AND REDUCED OFF-TARGET SITE EFFECTS
有权
基本信息:
- 专利标题： METHODS AND COMPOSITIONS FOR ENHANCED NUCLEASE-MEDIATED GENOME MODIFICATION AND REDUCED OFF-TARGET SITE EFFECTS
- 申请号：EP16819766.3 申请日：2016-12-06
- 公开(公告)号：EP3387134A1 公开(公告)日：2018-10-17
- 发明人： FRISCH, Ryan L.
- 申请人： Danisco US Inc.
- 申请人地址： 925 Page Mill Road Palo Alto, California 94304 US
- 专利权人： Danisco US Inc.
- 当前专利权人： Danisco US Inc.
- 当前专利权人地址： 925 Page Mill Road Palo Alto, California 94304 US
- 代理机构： Forrest, Graham Robert
- 优先权： US201562266051P 20151211
- 国际公布： WO2017100158 20170615
- 主分类号： C12N15/63
- IPC分类号： C12N15/63 ; C12N15/90
摘要：
Compositions and methods are provided for editing nucleotides or altering target sites in the genome of a cell. The methods and compositions employ a guide RNA/Cas endonuclease system and at least one component selected from the group consisting of (i) an inhibitor of end joining (NHEJ), (ii) an activator of homology-directed repair (HDR) or (iii) any one combination of (i) and (ii), to provide an effective system for editing nucleotides or altering target sites within the genome of a cell. The present disclosure also describes methods for editing a nucleotide sequence in the genome of a microbial cell employing a guide RNA/Cas endonuclease system and at least one component selected from the group consisting of (i) an inhibitor of NHEJ, (ii) an activator of HDR, or (iii) any one combination of (i) and (ii), wherein said microbial cell has reduced or no off-target site effects.