发明公开
EP3302450A1 AMINE PEGYLATION METHODS FOR THE PREPARATION OF SITE-SPECIFIC PROTEIN CONJUGATES
审中-公开
基本信息:
- 专利标题： AMINE PEGYLATION METHODS FOR THE PREPARATION OF SITE-SPECIFIC PROTEIN CONJUGATES
- 专利标题（中）：用于制备特定于位点的蛋白质缀合物的胺PEG化方法
- 申请号：EP16803982.4 申请日：2016-05-19
- 公开(公告)号：EP3302450A1 公开(公告)日：2018-04-11
- 发明人： ROSENDAHL, Mary S. , MANTRIPRAGADA, Sankaram B.
- 申请人： Antriabio, Inc.
- 申请人地址： 1450 Infinite Drive Louisville, Colorado 80027 US
- 专利权人： Antriabio, Inc.
- 当前专利权人： Antriabio, Inc.
- 当前专利权人地址： 1450 Infinite Drive Louisville, Colorado 80027 US
- 代理机构： Gill Jennings & Every LLP
- 优先权： US201562170933P 20150604
- 国际公布： WO2016196017 20161208
- 主分类号： A61K31/00
- IPC分类号： A61K31/00 ; C07K14/62 ; C07K14/00
摘要：
Examples include a method of making a protein-PEG conjugate. The method may include providing an aqueous protein solution. The aqueous protein solution may include a protein, a pH buffer, and a chelating agent. The chelating agent may be chosen from the group consisting of an aminopolycarboxylic acid, a hydroxyaminocarboxylic acid, an N-substituted glycine, 2-(2-amino-2-oxocthyl) aminoethane sulfonic acid (BES), and deferoxamine (DEF). The method may also include introducing sodium cyanoborohydride and a methoxy polyethylene glycol aldehyde to the aqueous protein solution. The sodium cyanoborohydride in the methoxy polyethylene glycol aldehyde may have a molar ratio ranging from about 5:1 to about 1.5:1. The method may further include reacting the methoxy polyethylene glycol aldehyde with the protein to form the protein-PEG conjugate. The pH buffer may maintain a pH of the aqueous protein solution ranging from 4.0 to 4.4 during the reaction.
IPC结构图谱:
| A | 人类生活必需 |
| --A61 | 医学或兽医学；卫生学 |
| ----A61K | 医用、牙科用或梳妆用的配制品 |
| ------A61K31/00 | 含有机有效成分的医药配制品 |