基本信息:
- 专利标题： BIOLOGICAL INDICATOR
- 专利标题（中）：生物指标
- 申请号：EP15747598.9 申请日：2015-06-16
- 公开(公告)号：EP3206723A1 公开(公告)日：2017-08-23
- 发明人： FRANCISKOVICH, Phillip P. , CREGGER, Tricia A. , YIRAVA, William A. , BURKE, Peter A.
- 申请人： American Sterilizer Company
- 申请人地址： 5960 Heisley Road Mentor, OH 44060 US
- 专利权人： American Sterilizer Company
- 当前专利权人： American Sterilizer Company
- 当前专利权人地址： 5960 Heisley Road Mentor, OH 44060 US
- 代理机构： Ström & Gulliksson AB
- 优先权： US201414512708 20141013
- 国际公布： WO2016060714 20160421
- 主分类号： A61L2/28
- IPC分类号： A61L2/28 ; C12M1/34 ; C12Q1/68
摘要：
This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
摘要（中）：
本发明涉及由组合物衍生的生物指示物，所述组合物包含：包含孢子形成细菌的宿主生物体; 用于产生指示酶的报道基因; 一个调控基因; 以及用于将报道基因和调控基因插入宿主生物体的载体; 宿主生物在其基因组中携带可转座的遗传元件，用于在调控基因中插入插入序列; 插入序列包含转座酶，一对末端反向重复序列和至少一个用于表达转座酶的开放阅读框。 该载体可以被宿主生物吸收。 插入序列可以插入调节基因中。 宿主生物可以进行孢子形成生物指示物。 公开了使用生物指示剂的方法和设备。