EP3161150B1 QUANTIFICATION METHODS TO DETERMINE INITIAL TEMPLATE CONCENTRATION BY MEANS OF ASYMMETRIC AMPLIFICATION
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基本信息:
- 专利标题： QUANTIFICATION METHODS TO DETERMINE INITIAL TEMPLATE CONCENTRATION BY MEANS OF ASYMMETRIC AMPLIFICATION
- 专利标题（中）：用不对称放大法确定初始模板浓度的量化方法
- 申请号：EP15733515.9 申请日：2015-06-29
- 公开(公告)号：EP3161150B1 公开(公告)日：2018-04-18
- 发明人： COBB, Ben
- 申请人： Epistem Limited
- 申请人地址： 48 Grafton Street Manchester M13 9XX GB
- 专利权人： Epistem Limited
- 当前专利权人： Epistem Limited
- 当前专利权人地址： 48 Grafton Street Manchester M13 9XX GB
- 代理机构： Williams, Gareth Owen
- 优先权： GB201411567 20140630
- 国际公布： WO2016001641 20160107
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
Described is a method for quantifying nucleic acid in a nucleic acid amplification reaction, in which an asymmetric nucleic acid amplification reaction is performed on a sample such that double stranded nucleic acid product is generated in an initial stage of the reaction, and single stranded nucleic acid product is generated in a subsequent stage of the reaction once a limiting primer is exhausted. Relative amounts of double stranded nucleic acid product and single stranded nucleic acid product produced are then detected by means of a melt curve analysis. The ratio of double stranded product peak height to single stranded product peak height may then be used to quantify the amount of starting template based on the ratio of double stranded product peak height to single stranded product peak height.