基本信息:
- 专利标题： DETECTION OF NUCLEIC ACIDS BY STRAND INVASION BASED AMPLIFICATION
- 申请号：EP14800105.0 申请日：2014-11-21
- 公开(公告)号：EP3071707B1 公开(公告)日：2018-10-31
- 发明人： EBOIGBODIN, Kevin , BRUMMER, Mirko
- 申请人： Orion Diagnostica Oy
- 申请人地址： Koivu-Mankkaan tie 6 B 02200 Espoo FI
- 专利权人： Orion Diagnostica Oy
- 当前专利权人： AIDIAN OY, FI
- 当前专利权人地址： AIDIAN OY, FI
- 代理机构： J A Kemp
- 优先权： EP13194118 20131122
- 国际公布： WO2015075198 20150528
- 主分类号： C12Q1/6844
- IPC分类号： C12Q1/6844 ; C12Q1/6818
摘要：
A method for detecting a target nucleic acid sequence in a sample in the presence of at least protein capable of binding to single-stranded DNA is provided, comprising contacting said sample with at least one oligonucleotide probe comprising a fluorophore, a quencher and a region complementary to said target nucleic acid sequence. The sequence of the oligonucleotide probe comprises at least 20% RNA nucleotides, modified RNA nucleotides and/or PNA nucleotides.