![ALKALINE PROTEASE VARIANTS](/ep/2018/12/12/EP3061817B1/abs.jpg.150x150.jpg)
基本信息:
- 专利标题: ALKALINE PROTEASE VARIANTS
- 申请号:EP16163320.1 申请日:2010-04-26
- 公开(公告)号:EP3061817B1 公开(公告)日:2018-12-12
- 发明人: TOHATA, Masatoshi , OKUDA, Mitsuyoshi , SATO, Tsuyoshi , ENDO, Keiji , SAEKI, Katsuhisa
- 申请人: KAO CORPORATION
- 申请人地址: 14-10, Nihonbashi Kayabacho 1-chome, Chuo-ku, Tokyo 103-8210 JP
- 专利权人: KAO CORPORATION
- 当前专利权人: KAO CORPORATION
- 当前专利权人地址: KAO CORPORATION
- 代理机构: Vossius & Partner Patentanwälte Rechtsanwälte mbB
- 优先权: JP2009110792 20090430; JP2009110793 20090430; JP2010093307 20100414; JP2010093339 20100414
- 主分类号: C12N9/54
- IPC分类号: C12N9/54 ; C11D3/386
摘要:
An alkaline protease variant derived from an alkaline protease consisting of an amino acid sequence represented by SEQ ID NO: 2 or consisting of an amino acid sequence having an identity of 90% or more therewith, which variant has mutations wherein one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of the amino acid sequence represented by SEQ ID NO: 2, or at positions corresponding thereto are substituted with the following amino acid residues: (a) or a position corresponding thereto: Typ, Leu, Val, Ile, Met, Tyr, Gln, Lys, Thr, Phe, Arg, Ser, Cys, Ala, or His; (b) or a position corresponding thereto: Glu, Met, Asp, Val, Gln, Arg, Cys, Trp, Ala, or Phe; (c) or a position corresponding thereto: Met, Glu, Arg, Val, Lys, Phe, Tyr, Ile, His, Asp, or Cys; (d) or a position corresponding thereto: Trp; (e) or a position corresponding thereto: His, Trp, Ser, or Leu; (f) or a position corresponding thereto: Ala, Glu, Gln, Ser, Cys, Gly, His, Lys, Arg, Met, or Asn; (g) or a position corresponding thereto: Ala, Ser, or Cys; (h) or a position corresponding thereto: Glu, Asp, Cys, Val, Thr, Pro, His, Ile, Trp, Ser, Asn, Lys, or Arg; (i) or a position corresponding thereto: Trp, Val, Thr, Leu, Ile, Cys, Glu, Lys, Tyr, Arg, Phe, Gln, Met, Pro, Asp, Asn, His, or Ser; and (j) or a position corresponding thereto: Arg, Gly, Ser, Lys, Gln, Thr, His, Ala, Cys, or Val.
公开/授权文献:
- EP3061817A1 ALKALINE PROTEASE VARIANTS 公开/授权日:2016-08-31