发明公开
EP2942353A1 Methods of reducing level of one or more impurities in a sample during protein purificaton
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基本信息:
- 专利标题： Methods of reducing level of one or more impurities in a sample during protein purificaton
- 专利标题（中）：法降低的一个或多个污染的程度在样本期间蛋白质清洁
- 申请号：EP15159525.3 申请日：2012-08-09
- 公开(公告)号：EP2942353A1 公开(公告)日：2015-11-11
- 发明人： Bian, Nanying , Gillespie, Christopher , Stone, Matthew , Kozlov, Mikhail , Chen, Jie , Siwak, Martin
- 申请人： EMD Millipore Corporation
- 申请人地址： 290 Concord Road Billerica, MA 01821 US
- 专利权人： EMD Millipore Corporation
- 当前专利权人： EMD MILLIPORE CORPORATION
- 当前专利权人地址： EMD MILLIPORE CORPORATION
- 代理机构： Suèr, Steven Johannes
- 优先权： US201161575349P 20110819; US201261666240P 20120629
- 主分类号： C07K1/16
- IPC分类号： C07K1/16 ; C07K1/20 ; B01D15/26 ; B01J20/282
摘要：
A flow-through process for purifying a target molecule from a Protein A eluate comprising the steps of:(i) contacting the eluate recovered from a Protein A chromatography column with activated carbon, (ii) contacting the flow-through sample from step (i) with an anion exchange chromatography media, (iii) contacting the flow-through sample from step (ii) with a cation exchange chromatography media and (iv) obtaining the flow-through sample from step (iii) comprising the target molecule, wherein the eluate flows continuously through steps (i)-(iii) and wherein level of one or more impurities in the flow-through sample after step (iii) is lower than the level in the eluate in step (i). The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely effecting the yield of the desired protein product.
摘要（中）：
（i）使从蛋白质回收用活性炭A层析柱的洗脱液，（ii）从步骤接触流过的样品（I：用于从蛋白质纯化目标分子A洗脱液，包括以下步骤阿流通方法 ）用阴离子交换色谱介质，（ⅲ）使来自步骤的流过样品（ii）用阳离子交换色谱介质以及（iv）从步骤（iii）包含所述目标分子流过的样品，worin的 （iii）和worin在流过样品AfterStep的一种或多种杂质的水平（ⅲ）比在步骤（i）中的洗脱物中的水平下 - 通过洗脱液步骤（i）连续地流动。 本发明提供了新颖的和相配合某些类型的碳质材料的，并导致有效的和选择性的除去某些不希望的杂质的不期望的蛋白质产物的产率产生不利影响改善的蛋白质纯化方法。