基本信息:
- 专利标题： NUCLEIC ACID ANALYSIS METHOD
- 专利标题（中）：核酸分析方法
- 申请号：EP12815243.6 申请日：2012-05-16
- 公开(公告)号：EP2735618B1 公开(公告)日：2017-12-06
- 发明人： SAITO, Toshiro , HAMASAKI, Koshin , TAKAHASHI, Satoshi , MAESHIMA, Muneo , IMAI, Kyoko , IMAI, Kazumichi , TAO, Ryuji
- 申请人： Hitachi High-Technologies Corporation
- 申请人地址： 24-14 Nishishimbashi 1-chome Minato-ku Tokyo 105-8717 JP
- 专利权人： Hitachi High-Technologies Corporation
- 当前专利权人： HITACHI HIGH-TECH CORPORATION, JP
- 当前专利权人地址： HITACHI HIGH-TECH CORPORATION, JP
- 代理机构： Moore, Graeme Patrick
- 优先权： JP2011157399 20110719
- 国际公布： WO2013011611 20130124
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12M1/34 ; C12N15/09
摘要：
The object of the present invention is to provide a convenient method for nucleic acid analysis, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provide is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. The present invention enables nucleic acids to be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.
公开/授权文献:
- EP2735618A1 NUCLEIC ACID ANALYSIS METHOD AND ANALYSIS APPARATUS 公开/授权日：2014-05-28