基本信息:
- 专利标题： BIOPROBES AND METHODS OF USE THEREOF
- 专利标题（中）：生物探针及使用方法
- 申请号：EP12744938.7 申请日：2012-02-06
- 公开(公告)号：EP2673381A2 公开(公告)日：2013-12-18
- 发明人： KELLEY, Shana, O. , FANG, Zhichao , VASILYEVA, Elizaveta
- 申请人： The Governing Council of the University of Toronto
- 申请人地址： 27 King's College Circle Toronto, ON M5S 1A1 CA
- 专利权人： The Governing Council of the University of Toronto
- 当前专利权人： The Governing Council of the University of Toronto
- 当前专利权人地址： 27 King's College Circle Toronto, ON M5S 1A1 CA
- 代理机构： Curran, Clair
- 优先权： US201161440336P 20110207
- 国际公布： WO2012109157 20120816
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C07H21/04 ; G01N33/50
摘要：
Disclosed are biomolecule based bioprobes that exhibit improved water solubility and monolayer-forming properties with substantially little or no aggregation that can appreciably interfere with binding of the bioprobes to a target nucleotide. The bioprobes may be used in conjunction with a suitable reporter system to detect very small quantities of biological markers. The bio-probes comprise a nucleobase sequence capable of hybridizing to a target nucleotide; and at least one charged functional group attached to said nucleobase sequence. Also disclosed are biosensors, and sensing devices that comprise the bio-probe. Further disclosed are suitable electrochemical reporter systems for use with bioprobes. Methods of use of these devices and probes, including for the detection of target biomarkers, including biomarkers for cancer cells or pathogens, are also included.