基本信息:
- 专利标题： ADVANCED PATHOGEN DETECTION AND SCREENING
- 专利标题（中）：扩展的证明和扩展的疾病筛查
- 申请号：EP10781389 申请日：2010-06-01
- 公开(公告)号：EP2435587A4 公开(公告)日：2012-10-31
- 发明人： ICENHOUR CRYSTAL , COLEMAN ELIZABETH J , LOYAL BRIAN V
- 申请人： PHTHISIS DIAGNOSTICS
- 专利权人： PHTHISIS DIAGNOSTICS
- 当前专利权人： PHTHISIS DIAGNOSTICS
- 优先权： US18236209 2009-05-29
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12N15/11
摘要：
Disclosed is a rapid, dual purpose, PCR-based method for identifying two or more pathogens, including Giardia and/or Cryptosporidium, in an extracted sample, such as stool or environmental (soil, water) isolates, in individual real-time PCR reactions. This method is of particular utility for clinical, veterinary, and environmental testing applications. The present methods are more sensitive than conventional ELISA or IFA-based methods of detection. An internal control (IC) for use in the PCR based nucleic acid detection method is also disclosed.