发明公开
EP2334827A1 METHOD FOR CHROMOGENIC DETECTION OF TWO OR MORE TARGET MOLECULES IN A SINGLE SAMPLE
有权
基本信息:
- 专利标题： METHOD FOR CHROMOGENIC DETECTION OF TWO OR MORE TARGET MOLECULES IN A SINGLE SAMPLE
- 专利标题（中）：检测方法的显色两个或多个目标分子在单个样本
- 申请号：EP09791784.3 申请日：2009-08-21
- 公开(公告)号：EP2334827A1 公开(公告)日：2011-06-22
- 发明人： GNIEWEK, Richard , FARRELL, Michael , NITTA, Hiroaki , LEHRKAMP, Megan , KOSMEDER, Jerome , BIENIARZ, Christopher , KELLY, Brian, Daniel , GROGAN, Thomas , GAIRE, Fabien , PADILLA, Mary
- 申请人： Ventana Medical Systems, Inc.
- 申请人地址： 1910 E. Innovation Park Drive Tucson, Arizona 85755 US
- 专利权人： Ventana Medical Systems, Inc.
- 当前专利权人： Ventana Medical Systems, Inc.
- 当前专利权人地址： 1910 E. Innovation Park Drive Tucson, Arizona 85755 US
- 代理机构： Gowshall, Jonathan Vallance
- 优先权： US189752P 20080822
- 国际公布： WO2010022332 20100225
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; G01N33/58
摘要：
The present invention provides a method and kit for detection of two or more target molecules in a single tissue sample, such as for gene and protein dual detection in a single tissue sample. Methods comprise treating a tissue sample with a first binding moiety that specifically binds a first target molecule. Methods further comprise treating the tissue sample with a solution containing a soluble electron-rich aromatic compound prior to or concomitantly with contacting the tissue sample with a hapten-labeled binding moiety and detecting a second target molecule. In one example, the first target molecule is a protein and the second is a nucleic acid sequence, the first target molecule being detected by immunohistochemistry and the second by in situ hybridization. The disclosed method reduces background due to non-specific binding of the hapten-labeled specific binding moiety to an insoluble electron rich compound deposited near the first target molecule.