基本信息:
- 专利标题： Reactive Heterocycle-Substituted 7-Hydroxycoumarins and Their Conjugates
- 专利标题（中）：反应性的杂环取代的7-羟基香豆素和它们的共轭
- 申请号：EP10158457.1 申请日：2010-03-30
- 公开(公告)号：EP2236508A1 公开(公告)日：2010-10-06
- 发明人： Diwu, Zhenjun , Dubrovsky, Timothy , Abrams, Barnaby , Liao, Jinfang , Meng, Qinglin
- 申请人： Becton, Dickinson and Company
- 申请人地址： 1 Becton Drive Franklin Lakes, NJ 07417 US
- 专利权人： Becton, Dickinson and Company
- 当前专利权人： Becton, Dickinson and Company
- 当前专利权人地址： 1 Becton Drive Franklin Lakes, NJ 07417 US
- 代理机构： von Kreisler Selting Werner
- 优先权： US165862P 20090401
- 主分类号： C07D405/04
- IPC分类号： C07D405/04 ; C07D405/14 ; C07D409/04 ; C07D409/14 ; C07D417/04 ; C07D417/14 ; A61K49/00
摘要：
Chemically-reactive, water-soluble, heterocycle-substituted 7-hydroxycoumarin dyes, their bioconjugates and uses are described. The conjugates derived from reactive heterocycle-substituted 7-hydroxycoumarin dyes are used for analyzing biological compounds. These heterocycle-substituted 7-hydroxycoumarin dyes are particularly useful as fluorescent labels for biopolymer detection reagents, such as antibodies or nucleic acid probes. The dye-antibody conjugates of the invention are particularly useful for analyzing analytes using a flow cytometer equipped with a violet laser as an excitation source due to their strong absorption at 405 nm and high fluorescence quantum yield.
公开/授权文献:
IPC结构图谱:
| C | 化学；冶金 |
| --C07 | 有机化学 |
| ----C07D | 杂环化合物 |
| ------C07D405/00 | 杂环化合物，含有1个或多个以氧原子作为仅有的杂环原子的环，且含有1个或多个以氮原子作为仅有的杂环原子的环 |
| --------C07D405/02 | .含有两个杂环 |
| ----------C07D405/04 | ..被环原子—环原子的键直接连接的 |