基本信息:
- 专利标题： ENHANCED IN VITRO SYNTHESIS OF ACTIVE PROTEINS CONTAINING DISULFIDE BONDS
- 专利标题（中）：增强了资产的体外合成，二硫键含有蛋白质
- 申请号：EP01968701.1 申请日：2001-09-06
- 公开(公告)号：EP1315826A1 公开(公告)日：2003-06-04
- 发明人： SWARTZ, James, Robert , KIM, Dong-Myung
- 申请人： THE BOARD OF TRUSTEES OFTHE LELAND STANFORD JUNIOR UNIVERSITY
- 申请人地址： 900 Welch Road,Suite 350 Stanford, CA 94304 US
- 专利权人： THE BOARD OF TRUSTEES OFTHE LELAND STANFORD JUNIOR UNIVERSITY
- 当前专利权人： THE BOARD OF TRUSTEES OFTHE LELAND STANFORD JUNIOR UNIVERSITY
- 当前专利权人地址： 900 Welch Road,Suite 350 Stanford, CA 94304 US
- 代理机构： Brasnett, Adrian Hugh
- 优先权： US230381P 20000906
- 国际公布： WO02020818 20020314
- 主分类号： C12P21/06
- IPC分类号： C12P21/06 ; A61K38/00 ; C07K1/00
摘要：
Compositions and methods are provided for the enhanced in vitro synthesis of polypeptides containing disulfide bonds. In order to improve the performance of in vitro protein synthesis reactions, pre-treatment and redox buffering of the reaction mix is performed in order to optimize the redox potential. Exogenous enzymes that enhance protein folding and disulfide bond formation may also be added to the reaction.
公开/授权文献:
IPC结构图谱:
| C | 化学；冶金 |
| --C12 | 生物化学；啤酒；烈性酒；果汁酒；醋；微生物学；酶学；突变或遗传工程 |
| ----C12P | 发酵或使用酶的方法合成目标化合物或组合物或从外消旋混合物中分离旋光异构体 |
| ------C12P21/00 | 肽或蛋白质的制备 |
| --------C12P21/06 | .由肽键水解产生，如水解产物 |