基本信息:
- 专利标题： EXPANSION OF STEM AND PROGENITOR CELLS BY BETA-CATENIN
- 专利标题（中）：STEM膨胀和细胞PREβ-连环蛋白
- 申请号：EP01903087.3 申请日：2001-01-17
- 公开(公告)号：EP1248519A1 公开(公告)日：2002-10-16
- 发明人： REYA, Tannishtha , NUSSE, Roeland , WEISSMAN, Irving, L.
- 申请人： THE BOARD OF TRUSTEES OFTHE LELAND STANFORD JUNIOR UNIVERSITY
- 申请人地址： 900 Welch Road,Suite 350 Stanford, CA 94304 US
- 专利权人： THE BOARD OF TRUSTEES OFTHE LELAND STANFORD JUNIOR UNIVERSITY
- 当前专利权人： THE BOARD OF TRUSTEES OFTHE LELAND STANFORD JUNIOR UNIVERSITY
- 当前专利权人地址： 900 Welch Road,Suite 350 Stanford, CA 94304 US
- 代理机构： Brasnett, Adrian Hugh
- 优先权： US176786P 20000118
- 国际公布： WO01052649 20010726
- 主分类号： A01N1/02
- IPC分类号： A01N1/02 ; C12N5/00 ; C12N5/02 ; C12N7/00 ; C12N15/63 ; C12N15/86 ; C12P21/04 ; C12P21/06
摘要：
Mammalian progenitor or stem cells are expanded in vitro by increasing the levels of β-catenin in the cell. The expanded cells substantially maintain their original phenotype including the ability to give rise to multiple types of differentiated cells. The intracellular levels of β-catenin may be manipulated by providing exogenous β-catenin protein to the cell, or by introduction into the cell of a genetic construct encoding β-catenin. The β-catenin may be a wild-type or stabilized mutant form of the protein. Preferably the long term cell culture medium substantially lacks stromal cells and cytokines.