基本信息:
- 专利标题： LIGAND SCREENING AND DESIGN BY X-RAY CRYSTALLOGRAPHY
- 专利标题（中）：筛选由X射线结合对象和他们的设计
- 申请号：EP99911174.3 申请日：1999-03-05
- 公开(公告)号：EP1068531B1 公开(公告)日：2007-10-10
- 发明人： NIENABER, Vicki, L. , GREER, Jonathan , ABAD-ZAPATERO, Celerino , NORBECK, Daniel, W.
- 申请人： ABBOTT LABORATORIES
- 申请人地址： Chad 0377/AP6D-2, 100 Abbott Park Road Abbott Park, Illinois 60064-3500 US
- 专利权人： ABBOTT LABORATORIES
- 当前专利权人： ABBOTT LABORATORIES
- 当前专利权人地址： Chad 0377/AP6D-2, 100 Abbott Park Road Abbott Park, Illinois 60064-3500 US
- 代理机构： Modiano, Micaela Nadia
- 优先权： US36184 19980306
- 国际公布： WO1999045379 19990910
- 主分类号： G01N33/53
- IPC分类号： G01N33/53 ; C30B7/00
摘要：
X-ray crystallography can be used to screen compounds that are not known ligands of a target biomolecule for their ability to bind the target biomolecule. The method includes obtaining a crystal of a target biomolecule; exposing the target biomolecule crytal to one or more test samples; and obtaining an X-ray crystal diffraction pattern to determine whether a ligand/receptor complex is formed. The target is exposed to the test samples by either co-crystallizing a biomolecule in the presence of one or more test samples or soaking the biomolecule crystal in a solution of one or more test samples. In another embodiment, structural information from ligand/receptor complexes are used to design ligands that bind tighter, that bind more specifically, that have better biological activity or that have better safety profile. A further embodiment of the invention comprises identifying or designing biologically-active moieties by the instant process. In a further embodiment, a biomolecule crystal having an easily accessible active site is formed by co-crystallizing the biomolecule with a degradable ligand and degrading the ligand.
公开/授权文献:
- EP1068531A2 LIGAND SCREENING AND DESIGN BY X-RAY CRYSTALLOGRAPHY 公开/授权日：2001-01-17