The utility model discloses an air purification cyclone separator which comprises a separator main body, a purification device and an air pipeline, and the separator main body is provided with a separation space, and an air inlet, an air outlet and an ash discharge port which are connected with the separation space; wherein the purification device comprises a shell and a plurality of groups of unbalanced ion alternating generators, the shell is provided with a purification inlet and a purification outlet, and the plurality of groups of unbalanced ion alternating generators are arranged in theshell; one end of the air pipeline is connected to the air outlet, and the other end of the air pipeline is connected to the purification inlet. According to the air purifier, large-particle-size particulate matter in air can be filtered out through the separator body, then the pre-filtered air is guided into the purifying device through the air pipeline to eliminate odorous gas and inhalable particulate matter, and the purified gas carries negative oxygen ions beneficial to human health.