The utility model relates to the field of biochemistry. The centrifugal process on-line monitoring equipment comprises a machine body, the centrifuge at least comprises a centrifuge shell and a control unit, the device further comprises a base frame, a high-speed camera, a pair of monitoring cameras, three adjusting supports, a connecting sleeve, a wireless module and a data line. The base frame is fixed to the inner upper wall face of the centrifugal machine shell. The three adjusting supports are fixed to the lower surface of the base frame, the high-speed camera and the pair of second cameras are detachably connected to the lower portions of the adjusting supports, the control unit and the wireless module are detachably connected to the outer circumferential face of the centrifugal machine shell, a wiring hole is formed in the centrifugal machine shell, and the connecting sleeve is embedded in the wiring hole. According to the utility model, the wireless module can be added into a network, so that a worker can see the working condition of the centrifuge at other places and know the running state in real time, and the burden of the worker is reduced.