The utility model provides a buckle formula is frozen and is deposited frame for deposit cryopreserved box, it is long square frame structure to freeze to deposit to put up, including four support posts, the top of four support posts is fixed with the roof, be equipped with the handle on the roof, the bottom mounting of four support posts has the bottom plate, freeze and deposit an inside from thetop down and be equipped with a plurality of horizontally baffles, the outer fringe of baffle with the support post is fixed, the leading edge of roof the leading edge of bottom plate and being located it encloses and establishes formation to freeze two support posts of depositing a front end cryopreserved box deposits the entry, it passes through to deposit the entry formation multi -layer storage entry, each are separated to the baffle the one side of depositing the entry is equipped with the buckle, the buckle can be opened or the shutoff with opening and shutting deposit the entry. The utility model discloses simple structure, convenient to use, the automation mechanized operation of being convenient for simultaneously can effectively improve work efficiency.