The utility model discloses an anti -return colloidal gold chromatography detects card aims at providing a compact structure, can prevent effectively to treat that sample detecting influences the colloidal gold chromatography of testing result at the excessive speed because of flowing and detects the card, its technical essential: including bottom plate and the panel of lock on the bottom plate, be equipped with the detect reagent card in the lock space between bottom plate and panel, the bottom plate on be equipped with the draw -in groove that is used for placing the detect reagent card, the support piece who is used for supporting the detect reagent card, a plurality of crossbeams and the fixed unit of label in proper order, support piece is the domatic, and its top of slope is close to the crossbeam, the panel on be equipped with application of sample hole and the fixed strip corresponding with the detect reagent card to and and the corresponding observation window of crossbeam, and fix the corresponding label window of unit with the label, belong to external diagnosis auxiliary instrument technical field.