The utility model provides a tissue culture bottle, includes that tissue culture bottle bottle and tissue culture bottle cover, and the tissue culture bottle lid is divided into interior bottle lid and outer bottle lid, and interior bottle lid upper end lies in the outer blank and covers the end internal surface, is equipped with fixing device between interior bottle lid and the outer bottle lid, and the fixing device periphery has the ventilated membrane to the parcel, and interior bottle lid is fixed through the fixing device connection among lieing in outer bottle lid. Interior bottle lid inboard is equipped with the internal thread, and tissue culture bottle bottle bottleneck is equipped with the external screw thread, and interior bottle lid passes through threaded connection with the tissue culture bottle bottle. Fixing device is the fixed strip, and the fixed strip upper end is located the outer blank and covers the end internal surface. The fixed strip is rigid plastic. The fixed strip welds integratively with interior bottle lid and outer bottle lid. Leave certain clearance between interior bottle lid and the outer bottle lid. The utility model discloses beneficial effect: 1. The utility model discloses simple structure, low in manufacturing cost, 2. The utility model discloses beautifully, the sight is high, 3. The utility model discloses ventilation effect is good, and practicality is strong.