The utility model relates to a temperature and humidity controller detection instrument comprising an analog signal source part and a detection part. The analog signal source part comprises a temperature sensor and a humidity sensor. The temperature sensor and the humidity sensor are respectively connected with a temperature control switch and a humidity control switch. The temperature sensor and the humidity sensor are respectively arranged in a sealing type temperature simulation tube and a sealing type humidity simulation tube which are respectively independent. The light judgment loop of the detection part is formed in a way that a green indicating lamp and a red indicating lamp are respectively connected with a voltage relay. The temperature and humidity controller detection instrument is mainly applied to daily work to directly inspect, study and judge a temperature and humidity controller without limitation of ambient temperature and humidity, and the temperature and humidity controller can be detected under the normal state. Reliability of the temperature and humidity controller in use can be enhanced, equipment safety can be enhanced, use is easy, carrying is convenient and recycling use can be realized so that manpower and material resources can be saved, working efficiency can be enhanced and the energy conservation and environmental protection effects can be realized.