The utility model relates to the field of civil engineering and particularly relates to a device applicable to box girder section prefabrication construction rebar binding. The device comprises channel steels, I-shaped steels, angle steels and positioning clamps. An outer frame skeleton is formed by the channel steels, the whole outer frame skeleton is similar to a cuboid, and the I-shaped steels are horizontally and longitudinally distributed on the upper surfaces of the channel steels below and above the outer frame skeleton. The angle steels and the I-shaped steels are arranged in the same direction, the outer surfaces of the angle steels are welded on the upper surfaces of the I-shaped steels in a single-side mode, the lengths of the single sides of the cross sections of the angle steels are smaller than or equal to the lengths of cross sections of the upper surfaces of the I-shaped steels, and semicircular grooves are opened at intervals according to design in the sides of the angle steels, which are not welded. The positioning clamps are arranged at the front end and the rear end of the outer frame skeleton. By means of the device, rebar binding is high in efficiency and accuracy.