The utility model discloses a purification device of enramycin. The purification device comprises a pretreatment tank, a ceramic membrane group, a polyethersulfone ultrafiltration membrane group, a first-stage polyethersulfone nanofiltration membrane group, a second-stage polyethersulfone nanofiltration membrane group and a precipitation tank, wherein the pretreatment tank is provided with a fermentation solution feed pipeline, a hydrochloric acid feed pipeline, an organic solvent feed pipeline and a discharge pipeline, the discharge pipeline is connected with the ceramic membrane group, a filtrate outlet of the ceramic membrane group is communicated with the polyethersulfone ultrafiltration membrane group, the interception molecular weight of the polyethersulfone ultrafiltration membrane group is 30KD, the filtrate outlet of the polyethersulfone ultrafiltration membrane group is communicated with the first-stage polyethersulfone nanofiltration membrane group, the interception molecular weight of the first-stage polyethersulfone nanofiltration membrane group is 5KD, the filtrate outlet of the first-stage polyethersulfone nanofiltration membrane group is communicated with the second-stage polyethersulfone nanofiltration membrane group, the interception molecular weight of the second-stage polyethersulfone nanofiltration membrane group is 1KD, an intercepted solution outlet of the second-stage polyethersulfone nanofiltration membrane group is communicated with the precipitation tank, and the precipitation tank is provided with the organic solvent feed pipeline. According to the device disclosed by the utility model, enramycin crude products can be obtained by purification, and the device is in line with industrial production needs.