The utility model discloses a temperature control system of a multi-layer co-extruded membrane transfusion bag production line. The system comprises a central controller, heating tubes, thermocouples and temperature controllers, wherein the heating tubes and the thermocouples are respectively used for heating each mould and acquiring the temperature signal of each mould; each welding mould is independently provided with a temperature controller, and the heating tube and the thermocouple on the corresponding welding mould are respectively controlled by each temperature controller to work; and each temperature controller is connected with a central processing unit through a communication bus and works under the control of the central processing unit. According to the utility model, the defect that no concentrated arrangement and monitoring exist when a temperature control meter is used for controlling independently in the prior art is overcome, the problem that one controller is used for controlling a plurality of channels is also solved, the control precision of the mould temperature is improved, the temperature fluctuation range is controlled within +/- 0.5 DEG C, the processing quality of a transfusion bag is ensured, parameters of equipment are set more easily and more conveniently, and the monitoring on the production process is more intuitive.