The utility model discloses an iris stitching instrument which comprises an iris stitching fixator and a line knot pusher; the iris stitching fixator comprises a handle and a fixing rod which are connected; a Y-shaped branch is arranged at the tail end of the fixing rod; the line knot pusher comprises a handle and a pushing rod which are connected; a line knot retaining bolt is arranged at the tail end of the pushing rod; a boss is arranged at the tail end of the line knot retaining bolt; due to existence of the line knot retaining bolt and the boss, grooves are formed on both sides of the line knot retaining bolt; the iris stitching instrument comprises two appliances, namely the iris stitching fixator and the line knot pusher; the iris stitching fixator can reduce the degree that the iris is drawn when being sewn, and a needle can be convenient to thread into the iris; the line knot pusher facilitates the drawing of line ends and the pushing of line knots; and since the line knot pusher is blunt, the line knot pusher can be safer to get in or out an anterior chamber. The iris stitching instrument provided by the utility model is a special instrument for iris stitching surgeries, has the advantages of simple structure, convenience in use and good use effect, and is advised to be popularized and applied.