The utility model relates to an enzyme-linked immunization kit for detecting spectinomycin residual amount in animal derived food. The kit comprises: 96-hole enzyme label plate, enzyme-labeled second antibody, spectinomycin antibody working fluid, spectinomycin standard substance solutions of six different concentrations, substrate color development solution, stop solution, concentrated cleaning solution, concentrated composite solution, high concentration standard substance, plate covering film, self-sealing bag, specification and quality inspection report. A indirect competition ELISA method is adopted, spectinomycin coupling antigen is precoated on a micropore strip of the enzyme label plate, spectinomycin contained in a sample competes with the coupling antigen precoated on the micropore strip for the spectinomycin antibody, after the enzyme-labeled second antibody is added, the TMB substrate is used for developing color, the sample absorbance value is compared with the standard curve and then is multiplied by corresponding dilution multiple to obtain the content of the spectinomycin in the sample. Compared with an instrument analysis technology, the utility model has characteristics of convenient use and high sensitivity, and can play an important role in residual detection of spectinomycin in muscle and liver.