基本信息:
- 专利标题： LIM矿化蛋白剪接变体
- 专利标题（英）：Lim mineralization protein splice variants
- 申请号：CN200610084228.9 申请日：2000-04-28
- 公开(公告)号：CN1908170A 公开(公告)日：2007-02-07
- 发明人： S·D·博登 , G·A·哈尔
- 申请人： 埃默里大学
- 申请人地址： 美国佐治亚
- 专利权人： 埃默里大学
- 当前专利权人： 埃默里大学
- 当前专利权人地址： 美国佐治亚
- 代理机构： 中国国际贸易促进委员会专利商标事务所
- 代理人： 赵艳华
- 优先权： 60/132,021 1999.04.30 US
- 分案原申请号： 008088950
- 主分类号： C12N15/12
- IPC分类号： C12N15/12 ; C12N15/63 ; C12N15/65 ; C07K14/435 ; C07K16/18 ; A61K48/00
The present invention is directed to isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. The invention further provides vectors comprising nucleotide sequences that encode LMP, as well as host cells comprising those vectors. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic precursor cells with an isolated nucleic acid molecule comprising a nucleotide sequence encoding LIM mineralization protein. The transfection may occur ex vivo or in vivo by direct injection of virus or naked plasmid DNA. In a particular embodiment, the invention provides a method of fusing a spine by transfecting osteogenic precursor cells with an isolated nucleic acid molecule having a nucleotide sequence encoding LIM mineralization protein, admixing the transfected osteogenic precursor cells with a matrix and contacting the matrix with the spine. Finally, the invention relates to methods for inducing systemic bone formation by stable transfection of host cells with the vectors of the invention.
公开/授权文献:
- CN100441688C LIM矿化蛋白剪接变体 公开/授权日：2008-12-10