基本信息:
- 专利标题： 一种大肠杆菌热不稳定肠毒素的制备方法
- 专利标题（英）：Process for preparing heat-labile enterotoxin of E, coli
- 申请号：CN200610010732.4 申请日：2006-03-09
- 公开(公告)号：CN1821398A 公开(公告)日：2006-08-23
- 发明人： 井申荣 , 魏云林 , 林连兵 , 李光
- 申请人： 昆明理工大学
- 申请人地址： 云南省昆明市学府路昆明理工大学
- 专利权人： 昆明理工大学
- 当前专利权人： 昆明理工大学
- 当前专利权人地址： 云南省昆明市学府路昆明理工大学
- 代理机构： 云南协立专利事务所
- 代理人： 旃习涵; 普卫东
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C12N1/19 ; C12N15/81 ; C12N15/31 ; C12P21/02 ; C12R1/84
The present invention discloses the preparation process of heat labile enterotoxin of E. coli, and the preparation process has intracellular expression or secretory expression of LT or LTB in yeast cell. Exogenously expressing LT and its mutant or subunit in eukaryotic yeast cell has greatly raised expression level. The present invention constitutes multicopy LT or LTB subunit expression kit, has increased gene dosage of LT or LTB in yeast, LT or LTB gene recombination following alcohol dehydrogenase promoter as the powerful promoter of Pichia yeast expression vector, methanol induced high expression of exogenous protein in yeast cell and thus raised expression level of LT and its mutant or LTB in yeast cell, simple post purification and safe clinical application. The present invention has low production cost and high target protein yield, and is significant in the scale production and clinical application of mucous membrane adjuvant.
公开/授权文献:
- CN100500842C 一种大肠杆菌热不稳定肠毒素的制备方法 公开/授权日：2009-06-17