The embodiment of the invention discloses an extraction method of globefish testis milt components. The extraction method of the globefish testis milt components includes the steps: globefish testes are pretreated, and scattered testis cells are obtained; ultrasonication is conducted on the scattered testis cells, and filtration and separation are carried out to obtain white precipitate; a set volume of concentrated hydrochloric acid is added to dissolve the white precipitate, and then a solution is formed; the solution passes through an ultrafiltration membrane, and then a trapped liquid anda filtrate are obtained separately; extraction is conducted on the trapped liquid, and then milt nucleic acids are obtained; and extraction is conducted on the filtrate, and then milt proteins are obtained. According to the extraction method of the globefish testis milt components, by utilizing the globefish testes as an extraction raw material, a large quantity of globefish viscus wastes producedby a globefish culture industry can be effectively utilized. Besides, according to the technical scheme, separation and extraction of the milt DNA and the milt proteins are achieved, thus, the availability of testis tissue can be improved, usage of surface active agents and organic solvents is also reduced, and therefore the extraction method of the globefish testis milt components is more environmentally friendly.