The invention relates to a pattern with a three-dimensional effect and a preparation method thereof. The pattern comprises printing ink and magnetic powder mixed together. The magnetic powder is arranged directionally in the printing ink. The pattern is printed by employing the printing ink containing the magnetic powder and is combined with a magnetic die, so that the magnetic powder in the pattern is arranged directionally in the printing ink, and therefore, the pattern has the three-dimensional effect. As the magnetic powder in the pattern is arranged directionally, the pattern cannot be scanned and copied, so that the pattern has a relatively good anti-counterfeiting property. The visual anti-counterfeiting pattern has a dynamic three-dimensional effect, a line refraction effect, a brightness and dumbness comparison effect, a depth-of-field three-dimensional effect and the like, and can be used in various packaging and printing fields such as cigarette packet printing, cosmetics box printing and gift box printing.