
基本信息:
- 专利标题: 检测非洲猪瘟病毒MGF360-505R基因的PCR引物组、试剂盒及检测方法
- 专利标题(英):PCR primer group, kit and detection method for detecting African swine fever virus MGF 360-505R gene
- 申请号:CN201911221389.1 申请日:2019-12-03
- 公开(公告)号:CN110724769A 公开(公告)日:2020-01-24
- 发明人: 勾红潮 , 李春玲 , 卞志标 , 孙铭飞 , 蔡汝健 , 宋帅 , 蒋智勇 , 李艳 , 杨冬霞 , 徐民生
- 申请人: 广东省农业科学院动物卫生研究所
- 申请人地址: 广东省广州市天河区五山路白石岗街21号
- 专利权人: 广东省农业科学院动物卫生研究所
- 当前专利权人: 广东省农业科学院动物卫生研究所
- 当前专利权人地址: 广东省广州市天河区五山路白石岗街21号
- 代理机构: 北京科亿知识产权代理事务所
- 代理人: 汤东凤
- 主分类号: C12Q1/70
- IPC分类号: C12Q1/70 ; C12Q1/6851 ; C12Q1/6848 ; C12N15/11 ; C12R1/93
The present invention discloses a PCR primer group, a kit and a detection method for detecting an African swine fever virus MGF 360-505R gene. The primer group comprises primers and a probe with nucleotide sequence shown as SEQ ID NO.1-3; the kit comprises the primers, the probe and a reaction reagent. When the kit is used, an S-type amplification curve appearing on an ROX channel is positive to the African swine fever virus, a non-S-type amplification curve is negative to the African swine fever virus infection, so that the kit can be expected to be used for differential diagnosis of a deletion strain. The provided kit can effectively prevent aerosol pollution, and is also simple to operate, low in cost, high in sensitivity and good in specificity, and suitable for export quarantine, foodsanitation and African swine fever virus identification and detection in livestock farms.