The invention discloses a night luminous warning belt for a floating hose and a use method thereof. The night luminous warning belt comprises a hose, wherein an electroluminescent film is coated outside the hose, and a reverse electro-osmosis layer is arranged between the electroluminescent film and the hose; the reverse electro-osmosis layer comprises cation exchange tubes and anion exchange tubes which are alternately arranged around the outer wall of the hose at intervals; two sides of the cation exchange tubes are connected with anode plates, the two sides of the anion exchange tubes are connected with cathode plates, all the anode plates and the cathode plates are positioned on the same circumference and are connected with the electroluminescent film, and each pair of adjacent anode plates and cathode plates correspond to each other; the electroluminescent film is coated with a filter layer, the filter layer is coated with a protective layer, and the electroluminescent film, the filter layer and the protective layer are all provided with porous structures. The invention aims to provide a night luminous warning belt which can be directly put into seawater together with the floating hose without directly using a luminous body material on the floating hose and a use method thereof.