The invention discloses a constant-temperature water bath device for semen detection, and belongs to the field of plastic processing equipment. The constant-temperature water bath device comprises a water bath barrel body, a container fixing mechanism, an oscillating mechanism, a stirring mechanism, a water supplementing mechanism and a lifting mechanism, wherein the water bath barrel body is arranged on the oscillating mechanism, the stirring mechanism is arranged at the bottom of the water bath barrel body, the lifting mechanism is arranged in the water bath barrel body, the container fixingmechanism is arranged on the lifting mechanism, the water supplementing mechanism is arranged on the outer wall of the water bath barrel body, a drainage pipeline is arranged at the bottom of the water bath barrel body, an electromagnetic valve is arranged on the drainage pipeline, and a heating pipe is arranged between the outer wall and the inner wall of the water bath barrel body. According tothe constant-temperature water bath device, a freezing pipe or a semen taking cup can be fixed through the container fixing mechanism, low-seepage liquid in a container is fully mixed with semen through the oscillating mechanism, the heat transfer speed in the water bath barrel body can be increased through the stirring mechanism, the outer wall of the container can be cleaned through the water supplementing mechanism, and automatic water supplementing can be carried out in the water bath barrel body through the water supplementing mechanism.