基本信息:
- 专利标题： 一种抗虫低酚棉花品种的育种方法
- 专利标题（英）：Method for breeding insect-resistant low gossypol cotton variety
- 申请号：CN201911022582.2 申请日：2019-10-25
- 公开(公告)号：CN110628789A 公开(公告)日：2019-12-31
- 发明人： 米换房 , 权月伟 , 唐光雷 , 翟雷霞 , 李继军 , 李文蕾
- 申请人： 邯郸市农业科学院
- 申请人地址： 河北省邯郸市邯山区农研路1号
- 专利权人： 邯郸市农业科学院
- 当前专利权人： 邯郸市农业科学院
- 当前专利权人地址： 河北省邯郸市邯山区农研路1号
- 代理机构： 北京睿博行远知识产权代理有限公司
- 代理人： 龚家骅
- 主分类号： C12N15/52
- IPC分类号： C12N15/52 ; C12N15/84 ; A01H5/00 ; A01H6/60
The invention provides a method for breeding an insect-resistant low gossypol cotton variety and belongs to the technical field of gene engineering. The method comprises the following steps: cloning aCADl-A gene to obtain CADl-A-l plasmids; constructing an RNAi expression vector of the CADl-A gene; performing genetic transformation on embryonic callus of transgenic Bt insect-resistant cotton by adopting an agrobacterium tumefaciens mediation method, wherein the CADl-A gene in the RNAi expression vector is driven by an alpha-globulin promoter, and the alpha-globulin promoter is modified with an Enh I enhancer. The method can inhibit methylation of the Bt gene promoter and the CADl-A gene seed specific promoter, so that inhibition on the Bt gene transcription level in a compound transgeniccrop can be remarkably reduced, reduction of the CADl-A gene transcription level can be promoted, toxin on embryonic callus can be reduced, the programmed death of transformed cells can be inhibited,and the transformation frequency can be improved.
公开/授权文献:
- CN110628789B 一种抗虫低酚棉花品种的育种方法 公开/授权日：2022-01-11