The invention belongs to the biotechnology field, and discloses a hexadecane degradation bacterial strain YJ1 and an application thereof. A degradation bacterial strain using hexadecane as a carbon source is obtained through separating and screening. According to the shape, the physiological characteristic, the Gramstaining reaction, the 16S rDNA gene sequencing analysis and phylogenetic analysisof the bacterial strain, the bacterial strain is identified to be nitration Brevibacillus nitrificans, and is named as YJ1. The bacterial strain is preserved in the China Committee for Culture Collection of Microorganisms in the August, 2019, and the preservation No. is CGMCC NO.18418. The bacterial strain can use the hexadecane as the only carbon source, and the hexadecane can be rapidly degraded. After the initial concentration of the hexadecane is cultured at the environment of 10mg/L for 15 days, the degradation rate can achieve 66.7%. The appropriate growth condition of the bacterial strain is that the temperature is 30 DEG C, the pH is 7 and the inoculum concentration is 10%. The bacterial strain has good degradation characteristic for the hexadecane, and cannot cause secondary pollution to environment.