The invention discloses monascus cheese and a preparation method thereof. The preparation method comprise the following steps: (1) fermenting raw milk by using a lactic acid bacteria starter and monascus; (2) preparing curd; (3) cutting the curd, stirring and standing the curd to obtain a curd block; (4) discharging whey; (5) placing the curd block into a mold for forming, regularly overturning the mold, demoulding the formed curd and soaking the demoulded curd in a salt solution to obtain cheese; (6) airing the cheese; and (7) spraying a natamycin solution on a surface of the cheese and ripening for 9 to 32 days. The preparation method avoids germs in the cheese environment from landing on the cheese surface, reduces contamination of the cheese by the germs, further improves product quality of the cheese, shortens production time of the cheese, and further enriches functions of the cheese in regulating sub-health state of a body such as regulating cholesterol, reducing blood sugar dueto the addition of monascus in the fermentation process.