The invention discloses a drug-loaded bacterial outer membrane vesicle, and a preparation method and application thereof, and particularly relates to a drug-loaded bacterial outer membrane vesicle prepared with the aid of liposome and a preparation method and application of the drug-loaded bacterial outer membrane vesicle. The application is in particular application in the preparation of antibacterial drugs. The invention provides the method for preparing a preparation containing the drug-loaded bacterial outer membrane vesicle, the method comprises the following steps of taking a preparationcontaining a drug-loaded liposome and a preparation containing a bacterial outer membrane vesicle as raw materials, and preparing to obtain the preparation containing the drug-loaded bacterial outermembrane vesicle. The invention further provides the method for preparing the drug-loaded bacterial outer membrane vesicle, the method comprises the following steps of taking a drug-loaded liposome and a bacterial outer membrane vesicle as raw materials, and preparing to obtain the drug-loaded bacterial outer membrane vesicle. The invention further provides the antibacterial drugs, including the preparation containing the drug-loaded bacterial outer membrane vesicle or the drug-loaded bacterial outer membrane vesicle. According to the drug-loaded bacterial outer membrane vesicle, the preparation method and application thereof, broad application prospects in the field of drug delivery and the field of antibacterial drug development achieved.