The invention relates to the technical field of healthcare products, in particular to a Chinese herbal composition for relieving stress and regulating emotion and a preparation method and applicationthereof. The Chinese herbal composition is prepared from, by weight, 1-10 parts of rhizoma chuanxiong, 2-20 parts of dried orange peels and 1-10 parts of rhizoma cyperi. Through compatibility of the three herbs, the efficacy of regulating qi and blood, activating blood circulation and relieving depression and pain is enhanced; the dried orange peels have the efficacy of eliminating dampness, removing phlegm, regulating qi and invigorating the spleen; through compatibility with rhizoma chuanxiong and rhizoma cyperi, the efficacy of regulating qi and relieving depression is enhanced, and meanwhile, the effect of invigorating the spleen to remove dampness is achieved. It is proved by experiments that the Chinese herbal composition can relieve rat delight deficiency caused by chronic stress and improve the neurotransmitter level. The composition takes plants applicable to pure natural healthcare food as raw materials and is simple in combination, safe, stable and suitable for long-term application.