The invention relates to a construction method for a one-way movable basin-type rubber support joint of an assembled long-span wood beam string structure, and the method comprises the following stepsof S1, simultaneously binding the bracket steel bars of a support and the steel bars of a main structural column; S2, measuring and positioning an embedded plate, manufacturing the embedded plate, andtemporarily fixing the embedded plate; S3, a support bracket supporting a template; pouring bracket concrete; removing a mold and cleaning the surface of the embedded plate; and S4, installing a unidirectional movable basin-type rubber support; manufacturing a gusset plate and a stiffening rib; welding a gusset plate and a stiffening rib into a whole; and compared with the prior art, the construction method can effectively ensure the construction quality of the one-way movable basin-type rubber support joint of the assembled long-span wood beam string structure, can synchronously and alternately perform a plurality of steps, effectively saves the construction period, improves the construction efficiency of construction enterprises, realizes the fabricated construction, and has stronger engineering practice application prospect and economic benefit.