基本信息:
- 专利标题： 一种多基因重组干酪乳酸杆菌、制备方法及应用
- 专利标题（英）：Multi-gene recombinant lactobacillus casei, preparation method and application
- 申请号：CN201910668557.5 申请日：2019-07-23
- 公开(公告)号：CN110358782A 公开(公告)日：2019-10-22
- 发明人： 杨霞 , 常洪涛 , 王新卫 , 刘红英 , 李永涛 , 赵军 , 陈陆 , 王坤芃 , 王川庆
- 申请人： 河南农业大学
- 申请人地址： 河南省郑州市金水区文化路95号
- 专利权人： 河南农业大学
- 当前专利权人： 河南农业大学
- 当前专利权人地址： 河南省郑州市金水区文化路95号
- 代理机构： 西安铭泽知识产权代理事务所
- 代理人： 李振瑞
- 主分类号： C12N15/62
- IPC分类号： C12N15/62 ; C12N15/74 ; C12R1/245
The invention relates to multi-gene recombinant lactobacillus casei, a preparation method and application. Target genes are separately connected into pMD18-T through T-A cloning and transformed into DH5alpha competent cells to obtain pMD18-T-S1, pMD18-T-GtxA-N and pMD18-T-flfA, the pMD18-T-S1, the pMD18-T-GtxA-N and the pMD18-T-flfA are separately used as templates for amplification to obtain three target fragments, the three target fragments are connected into one fragment, and pMG36e is amplified through PCR to make the pMG36e converted into a linearized vector. The fragment is connected with the linearized vector through a seamless cloning technology, the connected product is converted into XL1-Bule competent cells to obtain recombinant plasmids, and the recombinant plasmids are electrically converted into lactobacillus casei to obtain the recombinant lactobacillus casei.