The invention discloses a buckling and bend preventing high-voltage wiring harness layout structure for fine pin needle slots of a new energy vehicle, which structurally comprises a wiring harness collection base, a wiring box, a pin wiring harness slotted plate, a top cover plate, single control node holes, three-phase electric sockets, double-pin cap slots and main power supply sockets. According to the invention, the wiring harness collection base is applied to be matched with the pin wiring harness slotted plate, and pin needle wiring harnesses are inserted into I-shaped slot cylinders ina wiring harness slot cross frame and connected to a main cable in the wiring box by workers, so that the wiring harness collection base is conducted to achieve the effects of wiring, current collection and transmission. A needle tube of the wiring harness buckles and wraps from the head and tail ends through an arc plate cross-pulling ring and a covering and buckling corrugated plate, thereby forming an effect of pressing and protecting the needle tube body. The wire tube end at the needle tail part is taken in, thereby forming ductile pull-up deformation of the end for being matched with a rubber wire tube shell, reducing the stress of the needle head, and forming an attaching operation for preventing the slippage of a seal ring for the wire end. The stress-proof protection inner concavearc surface is strengthened in the middle section so as to achieve the effects of end quality protection of the wiring harnesses and accurate control of the new energy vehicles.