The invention discloses a preparation method for a tobacco endogenous Maillard reaction flavor and application of the tobacco endogenous Maillard reaction flavor in novel tobacco products. The preparation method for the tobacco endogenous Maillard reaction flavor comprises the following steps: (1) carrying out pretreatment on burley tobacco leaves and flue-cured tobacco leaves respectively and then placing in a high-pressure resistant PE bag, adding distilled water, performing ultra-high pressure treatment after stirring, filtering treatment liquid, and collecting filtrate, thereby obtaining ultra-high pressure treatment liquid of the tobacco leaves; (2) adding the ultra-high pressure treatment liquid of the tobacco leaves to a Maillard reactor, adding xylose, aspartate and a reaction solvent, adjusting the pH value, carrying out Maillard reaction, sealing and naturally cooling to obtain the reaction flavor. The flavor prepared by the invention can be added to low-temperature heated non-burning cigarettes, thereby helping to supplement the tobacco fragrance, to enhance the physiological satisfaction and oral sweetness of consumers, reducing the suction dryness and irritation, and improving the consumption comfort and tobacco simulation, and is further short in production cycle and high in preparation efficiency.