基本信息:
- 专利标题： 一种同时检测Exo I和TdT的电化学生物传感器的制备方法及其应用
- 专利标题（英）：Preparation method of electrochemical biosensor for simultaneously detecting Exo I and TdT and application thereof
- 申请号：CN201910096485.1 申请日：2019-01-21
- 公开(公告)号：CN109856211A 公开(公告)日：2019-06-07
- 发明人： 胡宇芳 , 胡丹丹 , 张青青 , 马少华 , 王邃 , 郭智勇
- 申请人： 宁波大学
- 申请人地址： 浙江省宁波市江北区风华路818号宁波大学
- 专利权人： 宁波大学
- 当前专利权人： 宁波大学
- 当前专利权人地址： 浙江省宁波市江北区风华路818号宁波大学
- 主分类号： G01N27/327
- IPC分类号： G01N27/327
The invention discloses a preparation method of an electrochemical biosensor for simultaneously detecting Exo I and TdT. Sulfhydryl DNA and a gold electrode surface form an Au-S covalent bond spontaneously by a mercapto part and the sulfhydryl DNA is fixed on the gold electrode surface. Polymerization of the 3'-OH tail of the TdT catalytic sulfydryl DNA and dCTP is carried out and a generated rich-C DNA long chain can used for forming AgNCs; and the TdT activity is detected by a dissolved voltammetric signal of Ag. Before TdT polymerization, Exo I is introduced and the sulfhydryl DNA can be hydrolyzed, so that the formation of AgNCs is affected finally; and thus the ExoI activity is detected. The electrochemical signal intensity can be affected by changing the ExoI or TdT concentration; and on the basis of the principle, a high-efficiency electrochemical biosensor for simultaneously detecting Exo I and TdT is prepared.
公开/授权文献:
- CN109856211B 一种同时检测Exo I和TdT的电化学生物传感器的制备方法及其应用 公开/授权日：2021-02-09
IPC结构图谱:
| G | 物理 |
| --G01 | 测量；测试 |
| ----G01N | 借助于测定材料的化学或物理性质来测试或分析材料 |
| ------G01N27/00 | 用电、电化学或磁的方法测试或分析材料 |
| --------G01N27/02 | .通过测试阻抗 |
| ----------G01N27/28 | ..电解池部件 |
| ------------G01N27/30 | ...电极，例如测试电极；半电池 |
| --------------G01N27/327 | ....生物化学电极 |