The invention discloses a portable continuous automatic preparation device and a portable continuous automatic preparation method for agar medium containing a temperature-sensitive active substance. The portable continuous automatic preparation device for agar medium containing a temperature-sensitive active substance comprises a thermal insulation and supporting housing unit, a multi-specification culture dish fixing and pushing unit, an electric control unit, a constant-temperature storage and agitating unit, an intermittent rotating unit, an automatic continuous pouring unit, and a disinfection and sterilization unit. According to the invention, the portable continuous automatic preparation device for agar medium containing a temperature-sensitive active substance is of a disassemblableportable design, the design is small, the portable continuous automatic preparation device for agar medium containing a temperature-sensitive active substance can be completely placed for operation under a spatially limited environment, such as a clean bench, the seven units operate in coordination, and can effectively ensure the constant-temperature quantitative pouring of an active substance, and thereby the requirement for the uniformity of medical and biological experiments is met. The invention fills the gap of continuous pouring devices for multiple specifications of agar media containing temperature-sensitive active substances applicable to laboratories in China.