The invention provides a low-temperature quick micro-freezing fresh-keeping technology of freshwater fishes. The low-temperature quick micro-freezing fresh-keeping technology adopts a high-tech biological technology, the ice crystal condition of organism cells under a low-temperature condition is studied, a low temperature and ultra-low temperature technology and a newly-invented micro-freezing fluid from natural components is applied to the freshwater fishes frozen for fresh keeping, an innovative freezing curve is applied, and the freshwater fishes are directly frozen in the micro-freezing fluid for fresh keeping. According to kinds and sizes of materials, freezing fresh keeping of the freshwater fishes is completed in 6-15 minutes or 15-30 minutes, which is presented in the respects that cell membranes of the freshwater fishes after the freezing fresh keeping are not frosted to be cracked and are under an organism micro-freezing condition; and then refrigerating is performed so as to maintain the freshwater fishes after the freezing fresh keeping fresh in quality. The low-temperature quick micro-freezing technology is an innovative micro-freezing fresh-keeping technology in theworld, the cells of the freshwater fishes are kept not cracked, and the transformation of polysaccharides substances in the freshwater fishes is improved; and when freezing fresh-keeping is performedon the fresh freshwater fishes in the shortest time according to the freezing curve, after the fresh freshwater fishes are stored under the refrigerating condition for within 12 months, the fresh quality of the fresh freshwater fishes is still maintained.